#3474 ROLE OF INTEGRIN, FIBRONECTIN, AND UPAR IN PODOCYTE-GLOMERULAR BASEMENT MEMBRANE INTERACTION UNDER FLUID SHEAR STRESS

Abstract Background and Aims Podocyte-glomerular basement membrane (GBM) interaction is the main component to maintain glomerular filtration barrier. Interestingly, podocyte-GBM not static, but dynamic according various podocyte conditions. Thus, we hypothesized that would be changed under stress conditions such as fluid shear (FSS). Here, aim explore change of specific integrin (Itg αVβ3), fibronectin, uPAR in Method We used conditionally immortalized human cells which were differentiated only (non-permissive) temperature (37°C). exposed podocytes 200 rpm FSS applied dish coating agents. observed cell morphology documented protein mRNA expressions Itg β3, FSS. Results Podocyte became more elongated with actin rearrangement Protein expression β3 did FSS, while activated increased from early phase (30 min after exposure FSS) (Figure 1). Fibronectin 2) 3) gradually Cell viability collagen-1 coated compared non-coated Conclusion This study demonstrates αVβ3 activated, fibronectin increase In addition, it shown extracellula matrix contributes stability. The results suggest αVβ3, may have roles podocyte's stability through modulation interaction.